Journal: Molecular Medicine

Article Title: α-1-Antitrypsin Gene Delivery Reduces Inflammation, Increases T-Regulatory Cell Population Size and Prevents Islet Allograft Rejection

doi: 10.2119/molmed.2011.00145

Figure Lengend Snippet: Circulating pEF-hAAT–derived hAAT modifies posttransplantation foxp3 Treg cell population. (A) Mice were grafted intraperitoneally with allogeneic skin tissue, and splenic Treg cell population size was assessed 7 d after transplantation. Control (nontransplanted mice): CT, background Treg cell population size in mice that were not injected by HD tail-vein injection (n = 6); pEF-hAAT, Treg cell population size from mice that were HD tail-vein injected with pEF-hAAT (n = 6). Transplantation (skin-tissue graft): PBS, allo-geneic skin-tissue transplantation into mice that were HD tail-vein injected with PBS (n = 6). pEF-hAAT, allogeneic skin-tissue transplantation into mice that were HD tail-vein injected with pEF-hAAT (n = 6). Treg cell population size was assessed by FACS after CD4-positive T-cell enrichment. Mean ± SEM; *P < 0.05. (B) Representative FACS analysis images (y axis: forward scatter [FSC]; x axis: Foxp3-GFP).

Article Snippet: After washing with a fluorescence-activated cell sorter (FACS) buffer (PBS containing 2% bovine serum albumin, 0.1% sodium azide and 0.1% EDTA, pH 7.4), splenocytes (1 × 10 6 cells per sample) were either stained with allophycocyanin (APC)-conjugated anti–mouse CD4 (BioLegend) or underwent magnetic bead enrichment for CD4- positive T cells (EasySep ® ; Stem-Cell Technologies, BC, Canada).

Techniques: Derivative Assay, Transplantation Assay, Control, Injection